再生医学领域有望通过激活人体的自然修复系统来为受损的器官和组织创造更有效的治疗方法。全球研究人员正在不断生产viable sample tissues，但是生物体内部有效性的例子较少。
A multidisciplinary team of researchers from leading academic institutions in Japan are working on3D生物打印小肝的产生。在最近的研究中发表在《杂志》上Scientific Reports，该小组展示了将其3D生物打印肝组织植入活大鼠的能力。
The successful procedure is a positive result for the further development of therapeutic treatments, and adds evidence to new ways spherical cell samples can be made.
3D比2D好
许多干细胞研究得出的结论是，在组织工程方面，3D大于2D。就京都大学和合作伙伴的这项研究而言，“球体的融合”即2D培养基中的肝细胞“无法形成受控或均匀的结构”。
“Furthermore, the spheroids did not fuse at the central area due to the lack of medium supply in static culture conditions.”
And so, “To overcome these problems, we used a new 3D printing technology to create a reproducible structure and enable culture circulation.”
Pin point accuracy
Rudimentary liver buds, also called spheroids, were made into tissue by3D生物打印的Kenzan方法。Kenzan方法中的肝细胞球体被放置在聚集在一起的一组针上。
在三天的时间里，球体融合在一起，因此可以从阵列中取出。之后，样品培养在孔上关闭，产生了针头，留下坚固，柔性的管。
The tube shaped specimen was made from approximately 500 spheroids, and measures 4 × 4 × 5mm.
Gif shows a test of the physical strength of the 3D bioprinted liver tube. Clip via Scientific Reports, Supplementary Materials
Successful transplantation
Once fabricated the liver buds were test transplanted into living mice in a number of different ways. In the method that stood out, the 3D bioprinted sample grafted into the mouse liver in the first 7 days after transplantation. This method, according to the authors, has two major advantages. First, “it does not involve any vascular obstructions.” Second “the presence of direct connection between the graft and the recipient’s liver parenchyma” i.e. the functional part of the organ, “would facilitate greater graft growth.”
此外，使用Kenzan方法，研究人员展示了他们认为是生产肝样组织的快速和可缩度的方法。他们评论说：“它允许在3D组织中制造精美的几何形状，并使用针形阵列系统固定形状，使3D打印后可以立即执行培养循环。”
Author affiliations
The Kenzan method is commercialized by biomedical technology firm Cyfuse. Tadashi Tamura, one of the co-authors of the study is employed by Cyfuse Biomedical in a research capacity.
其他作者包括京都大学的Yusuke Yanagi，Tomoaki Taguchi，Koichiro Yoshimaru，Toshiharu Matsuura，Makoto Hayashida，Kenichi Kohashi，Yoshinao Oda和Takayoshi Yamaza；Keio大学的Shin Enosawa和Eiji Kobayashi；和萨加大学的Koichi Nakayama。
用于医学研究等等like，，，，follow，，，，andsubscribeto 3D Printing Industry.在此处注册3D打印作业。
特色图像显示了九州大学研究中使用的Kenzan 3D Bioprinitng和植入过程。通过科学报告图像
